Molecular and Biochemical Techniques for Deciphering p53-MDM2 Regulatory Mechanisms

Molecular and Biochemical Techniques for Deciphering p53-MDM2 Regulatory Mechanisms

Blog Article

The p53 and Mouse double minute 2 (MDM2) proteins are hubs in extensive networks of interactions with multiple partners and functions.Intrinsically disordered regions help to adopt function-specific structural conformations in response to ligand binding and post-translational modifications.Different techniques have been used to dissect interactions of the p53-MDM2 pathway, in vitro, in vivo, and in situ each having its own advantages and disadvantages.This review uses the p53-MDM2 to show how different techniques can be employed, illustrating how Chillum a combination of in vitro and in vivo techniques is highly recommended to study the spatio-temporal location and dynamics of interactions, and to address their regulation mechanisms and functions.

By using well-established techniques in combination with more recent advances, it SHOPSTORM_HIDDEN_PRODUCT is possible to rapidly decipher complex mechanisms, such as the p53 regulatory pathway, and to demonstrate how protein and nucleotide ligands in combination with post-translational modifications, result in inter-allosteric and intra-allosteric interactions that govern the activity of the protein complexes and their specific roles in oncogenesis.This promotes elegant therapeutic strategies that exploit protein dynamics to target specific interactions.